Improved continuous-flow method for detemination of total serum hexosamines.
نویسندگان
چکیده
We describe an automated determination of serum hexosamine by the Elson-Morgan reaction, together with reagent modifications that minimiz interference from amino acids and sugars present in acid hydrolysates of sera. We used a novel 15-min autoclave procedure for the acid hydrolysis of sera before analysis to facilitate the detemination as compared to the 4-h hydrolysis used in the conventional manual method. Results correlated well (r = 0.906) with those obtained by the corresponding manual method.
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عنوان ژورنال:
- Clinical chemistry
دوره 22 8 شماره
صفحات -
تاریخ انتشار 1976